Identification, sequencing and expression of an integral membrane protein of the <i>trans</i>-Golgi network (TGN38)
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- J P Luzio
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
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- B Brake
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
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- G Banting
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
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- K E Howell
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
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- P Braghetta
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
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- K K Stanley
- European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, Federal Republic of Germany.
書誌事項
- 公開日
- 1990-08-15
- DOI
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- 10.1042/bj2700097
- 公開者
- Portland Press Ltd.
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説明
<jats:p>Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.</jats:p>
収録刊行物
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- Biochemical Journal
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Biochemical Journal 270 (1), 97-102, 1990-08-15
Portland Press Ltd.