Temporal and Compositional Characteristics of Salivary Protein Adsorption to Hydroxyapatite

DOI Web Site 被引用文献7件
  • M.S. Lamkin
    Department of Periodontology and Oral Biology, Boston University Medical Center, Center for Advanced Biomedical Research, 700 Albany St., Boston, Massachusetts 02118
  • A.A. Arancillo
    Department of Periodontology and Oral Biology, Boston University Medical Center, Center for Advanced Biomedical Research, 700 Albany St., Boston, Massachusetts 02118
  • E.G. Oppenheim
    Department of Periodontology and Oral Biology, Boston University Medical Center, Center for Advanced Biomedical Research, 700 Albany St., Boston, Massachusetts 02118

この論文をさがす

説明

<jats:p> Salivary proteins bind to enamel surfaces and hydroxyapatite in a highly selective manner. Numerous studies have identified these proteins as primarily proline-rich proteins, cystatins, statherin, and histatins. Previously, the hydroxyapatite-binding potential of these proteins had been characterized in systems consisting of singly purified protein and adsorbent. The purpose of this study was to investigate the adsorption of each protein in the presence of complete salivary secretion. Proteins, shown to adsorb to hydroxyapatite, were purified, biotinylated, and added back to the remaining proteins to form a series of reconstituted secretions. The adsorption of each biotinylated protein in the reconstituted secretion to hydroxyapatite was then measured as a function of time. Results indicated that three different adsorption patterns occur. A simple hyperbolic pattern is characteristic of amylase, glycosylated proline-rich protein (PRG), and cystatin. A faster adsorption process is observed for PRP-3, PRP-4, PIF-f, and statherin. A more complex pattern, exhibiting a rapid phase followed by a slower phase, is characteristic of PRP-1, PRP-2, PIF-s, and histatins. These results suggest that there are different adsorption processes involved in the binding of salivary proteins to hydroxyapatite. Two possible mechanisms are direct adsorption of protein to hydroxyapatite and indirect adsorption of protein by interacting with other proteins already bound to hydroxyapatite. </jats:p>

収録刊行物

被引用文献 (7)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ