Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis

Alice Grob, Pascal Roussel, Jane E. Wright, Brian McStay, Danièle Hernandez-Verdun, Valentina Sirri

doi:10.1242/jcs.042382

<jats:p>Sirtuins, also designated class III histone deacetylases, are implicated in the regulation of cell division, apoptosis, DNA damage repair, genomic silencing and longevity. The nucleolar Sirtuin7 (SIRT7) was reported to be involved in the regulation of ribosomal gene (rDNA) transcription, but there are no data concerning the regulation of SIRT7 during the cell cycle. Here we have analyzed the behavior of endogenous SIRT7 during mitosis, while rDNA transcription is repressed. SIRT7 remains associated with nucleolar organizer regions, as does the RNA polymerase I machinery. SIRT7 directly interacts with the rDNA transcription factor UBF. Moreover, SIRT7 is phosphorylated via the CDK1-cyclin B pathway during mitosis and dephosphorylated by a phosphatase sensitive to okadaic acid at the exit from mitosis before onset of rDNA transcription. Interestingly, dephosphorylation events induce a conformational modification of the carboxy-terminal region of SIRT7 before the release of mitotic repression of rDNA transcription. As SIRT7 activity is required to resume rDNA transcription in telophase, we propose that this conformational modification regulates onset of rDNA transcription.</jats:p>

Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis

書誌事項

この論文をさがす

説明

収録刊行物

被引用文献 (2)*注記

詳細情報詳細情報について

書き出し

問題の指摘

Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis

書誌事項

この論文をさがす

説明

収録刊行物

被引用文献 (2)*注記

詳細情報 詳細情報について

書き出し

問題の指摘

詳細情報詳細情報について