Strigolactone Biosynthesis in <i>Medicago</i> <i>truncatula</i> and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2
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- Wei Liu
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Wouter Kohlen
- Department of Plant Science, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Alessandra Lillo
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Rik Op den Camp
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Sergey Ivanov
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Marijke Hartog
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Erik Limpens
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Muhammad Jamil
- Department of Plant Science, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Cezary Smaczniak
- Plant Research International–Bioscience, Laboratory of Molecular Biology, 6708 PB Wageningen, The Netherlands
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- Kerstin Kaufmann
- Plant Research International–Bioscience, Laboratory of Molecular Biology, 6708 PB Wageningen, The Netherlands
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- Wei-Cai Yang
- Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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- Guido J.E.J. Hooiveld
- Division of Human Nutrition, Wageningen University, 6703 HD Wageningen, The Netherlands
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- Tatsiana Charnikhova
- Department of Plant Science, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Harro J. Bouwmeester
- Department of Plant Science, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- Ton Bisseling
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
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- René Geurts
- Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, 6708 PB Wageningen, The Netherlands
書誌事項
- 公開日
- 2011-10-01
- 権利情報
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- https://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1105/tpc.111.089771
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression after symbiotic signaling. However, legume NSP1 and NSP2 can be functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP1 and NSP2, indicating that both proteins are functionally conserved in higher plants. Here, we show that NSP1 and NSP2 are indispensable for strigolactone (SL) biosynthesis in the legume Medicago truncatula and in rice. Mutant nsp1 plants do not produce SLs, whereas in M. truncatula, NSP2 is essential for conversion of orobanchol into didehydro-orobanchol, which is the main SL produced by this species. The disturbed SL biosynthesis in nsp1 nsp2 mutant backgrounds correlates with reduced expression of DWARF27, a gene essential for SL biosynthesis. Rice and M. truncatula represent distinct phylogenetic lineages that split approximately 150 million years ago. Therefore, we conclude that regulation of SL biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. NSP1 and NSP2 are single-copy genes in legumes, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as SL biosynthesis in nonsymbiotic conditions.</jats:p>
収録刊行物
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- The Plant Cell
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The Plant Cell 23 (10), 3853-3865, 2011-10-01
Oxford University Press (OUP)