Direct detection of <i>Streptococcus mutans</i> in human dental plaque by polymerase chain reaction
書誌事項
- 公開日
- 1996-10
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1399-302x.1996.tb00184.x
- 公開者
- Wiley
この論文をさがす
説明
<jats:p> <jats:italic>Igarashi T, Yamamoto AA, Goto N. Direct detection of</jats:italic> Streptococcus mutans <jats:italic>in human dental plaque by polymerase chain reaction.</jats:italic></jats:p><jats:p> <jats:italic>Streptococcus mutans</jats:italic> is an etiological agent in human dental caries. A method for the detection of <jats:italic>S. mutans</jats:italic> directly from human dental plaque by polymerase chain reaction has been developed. Oligonucleotide primers specific for a portion of the dextranase gene (dexA) of <jats:italic>S. mutans</jats:italic> Ingbritt (serotype c) were designed to amplify a 1272‐bp DNA fragment by polymerase chain reaction. The present method specifically detected <jats:italic>S. mutans</jats:italic> (serotypes c, e and f), but none of the other mutans streptococci: <jats:italic>S. cricetus</jats:italic> (serotype a), <jats:italic>S. rattus</jats:italic> (serotype b), <jats:italic>S. sobrinus</jats:italic> (serotypes d and g), and <jats:italic>S. downei</jats:italic> (serotype h), other gram‐positive bacteria (16 strains of 12 species of cocci and 18 strains of 12 species of bacilli) nor gram‐negative bacteria (1 strain of 1 species of cocci and 20 strains of 18 species of bacilli). The method was capable of detecting 1 pg of the chromosomal DNA purified from <jats:italic>S. mutans</jats:italic> Ingbritt and as few as 12 colony‐forming units of <jats:italic>S. mutans</jats:italic> cells. The <jats:italic>S. mutans</jats:italic> cells in human dental plaque were also directly detected. Seventy clinical isolates of <jats:italic>S. mutans</jats:italic> isolated from the dental plaque of 8 patients were all positive by the polymerase chain reaction. These results suggest that the <jats:italic>dexA</jats:italic> polymerase chain reaction is suitable for the specific detection and identification of <jats:italic>S. mutans.</jats:italic></jats:p>
収録刊行物
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- Oral Microbiology and Immunology
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Oral Microbiology and Immunology 11 (5), 294-298, 1996-10
Wiley