Some enzymological properties of a hatching enzyme (Chorionase) isolated from the fresh-water teleost, Oryzias latipes
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説明
Abstract 1. 1. Some properties of an isolated hatching enzyme of the medaka, Oryzias latipes , were studied. 2. 2. Under the assay conditions, the enzyme was inhibited by high concentrations of Na + , K + , Ca 2+ and Mg 2+ , while it was slightly activated by them at low concentrations. The optimum pH of this enzyme was around 7·9. 3. 3. This enzyme was more stable in neutral or alkaline medium than in acid medium and was inhibited by ethylenediaminetetracetic acid but not by diisopropylfluorophosphate or monoiodoacetamide. 4. 4. A substrate specificity test using some synthetic substrates revealed that this enzyme was medially specific.
収録刊行物
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- Comparative Biochemistry and Physiology Part B: Comparative Biochemistry
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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 46 (3), 603-614, 1973-11
Elsevier BV
