Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5

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  • Illyce Nuñez
    Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States
  • Marbelys Rodriguez Pino
    Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States
  • David J Wiley
    Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States
  • Maitreyi E Das
    Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, United States
  • Chuan Chen
    Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States
  • Tetsuya Goshima
    National Research Institute of Brewing, Higashi-Hiroshima, Japan
  • Kazunori Kume
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Dai Hirata
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Takashi Toda
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Fulvia Verde
    Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States

Description

<jats:p>RNA-binding proteins contribute to the formation of ribonucleoprotein (RNP) granules by phase transition, but regulatory mechanisms are not fully understood. Conserved fission yeast NDR (Nuclear Dbf2-Related) kinase Orb6 governs cell morphogenesis in part by spatially controlling Cdc42 GTPase. Here we describe a novel, independent function for Orb6 kinase in negatively regulating the recruitment of RNA-binding protein Sts5 into RNPs to promote polarized cell growth. We find that Orb6 kinase inhibits Sts5 recruitment into granules, its association with processing (P) bodies, and degradation of Sts5-bound mRNAs by promoting Sts5 interaction with 14-3-3 protein Rad24. Many Sts5-bound mRNAs encode essential factors for polarized cell growth, and Orb6 kinase spatially and temporally controls the extent of Sts5 granule formation. Disruption of this control system affects cell morphology and alters the pattern of polarized cell growth, revealing a role for Orb6 kinase in the spatial control of translational repression that enables normal cell morphogenesis.</jats:p>

Journal

  • eLife

    eLife 5 e14216-, 2016-07-30

    eLife Sciences Publications, Ltd

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