Quantifying <i>E. coli</i> Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells

DOI Web Site 60 Citations
  • Yuichi Taniguchi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Paul J. Choi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Gene-Wei Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Huiyi Chen
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Mohan Babu
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.
  • Jeremy Hearn
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Andrew Emili
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.
  • X. Sunney Xie
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Description

<jats:title>Devil in the Detail</jats:title> <jats:p> Genetically identical cells in the same environment can show variation in gene expression that may cause phenotypic variation at the single-cell level. But how noisy are most genes? <jats:bold> Taniguchi <jats:italic>et al.</jats:italic> </jats:bold> (p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" page="533" related-article-type="in-this-issue" vol="329" xlink:href="10.1126/science.1188308">533</jats:related-article> ; see the Perspective by <jats:bold> <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="5991" page="518" related-article-type="in-this-issue" vol="329" xlink:href="10.1126/science.1194036">Tyagi</jats:related-article> </jats:bold> ) now report single-cell global profiling of both messenger RNA (mRNA) and proteins in <jats:italic>Escherichia coli</jats:italic> using a yellow fluorescent protein fusion library. As well as a common extrinsic noise in high-abundance proteins, large fluctuations were observed in low-abundance proteins. Remarkably, in single-cell experiments, mRNA and protein levels for the same gene were uncorrelated. </jats:p>

Journal

  • Science

    Science 329 (5991), 533-538, 2010-07-30

    American Association for the Advancement of Science (AAAS)

Citations (60)*help

See more

Details 詳細情報について

Report a problem

Back to top