Development and use of simple sequence repeat SSR markers in <i>Rubus</i> species

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<jats:title>Abstract</jats:title><jats:p>The isolation of polymorphic codominant microsatellite markers in <jats:italic>Rubus</jats:italic> and in particular red raspberry will provide a tool to investigate gene flow between cultivated and wild raspberries. Microsatellite loci were isolated by screening a <jats:italic>Pst</jats:italic>I size selected genomic library with AC<jats:sub>(13)</jats:sub> and AG<jats:sub>(13)</jats:sub>. Positive clones were sequenced and primer pairs designed to the sequences flanking identified SSRs. One primer of each pair was fluorescently labelled to facilitate polymerase chain reaction (PCR) product identification on an automated DNA sequencer. We describe 10 polymorphic microsatellite loci developed and demonstrate their usefulness in different <jats:italic>Rubus</jats:italic> species.</jats:p>

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