Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (<i>Bubalus bubalis</i>var.<i>indicus</i>) detected by PCR-RFLP
説明
<jats:title>Abstract</jats:title><jats:p>Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were used successfully to amplify the equivalent region in 34 Murrah and 36 Surti buffaloes selected at random. The 304 bp amplified product of the DRB3 gene was separately digested with<jats:italic>Bst</jats:italic>γI,<jats:italic>Hae</jats:italic>III and<jats:italic>Rsa</jats:italic>l enzymes. Digestion with<jats:italic>Bst</jats:italic>γI enzyme did not reveal any polymorphism and all animals showed a single restriction pattern, which corresponded exactly to the<jats:italic>Bst</jats:italic>γI pattern ‘b’ previously described for cattle. Digestion with<jats:italic>Hae</jats:italic>III enzyme resulted in five patterns, four of which corresponded to the<jats:italic>Hae</jats:italic>lll patterns previously reported in cattle. The new<jats:italic>Hae</jats:italic>III pattern was observed in both the breeds of buffaloes studied. The fragment analysis with<jats:italic>Rsa</jats:italic>I revealed 13 different patterns. All of these<jats:italic>Rsa</jats:italic>I patterns corresponded to the<jats:italic>Rsa</jats:italic>I patterns previously described for cattle. The high degree of similarity in the restriction fragment length polymorphism (RFLP) patterns of cattle and buffalo observed in the present study provide evidence for the strong conservation amongst other bovine species, of restriction sites previously reported in cattle.</jats:p>
収録刊行物
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- Animal Science
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Animal Science 70 (2), 221-226, 2000-04
Cambridge University Press (CUP)
