Cadmium and iron transport by members of a plant metal transporter family in <i>Arabidopsis</i> with homology to <i>Nramp</i> genes
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- Sébastien Thomine
- Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116; and Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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- Rongchen Wang
- Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116; and Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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- John M. Ward
- Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116; and Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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- Nigel M. Crawford
- Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116; and Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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- Julian I. Schroeder
- Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116; and Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
説明
<jats:p> Metal cation homeostasis is essential for plant nutrition and resistance to toxic heavy metals. Many plant metal transporters remain to be identified at the molecular level. In the present study, we have isolated <jats:italic>AtNramp</jats:italic> cDNAs from <jats:italic>Arabidopsis</jats:italic> and show that these genes complement the phenotype of a metal uptake deficient yeast strain, <jats:italic>smf1</jats:italic> . <jats:italic>AtNramp</jats:italic> s show homology to the <jats:italic>Nramp</jats:italic> gene family in bacteria, yeast, plants, and animals. Expression of <jats:italic>AtNramp</jats:italic> cDNAs increases Cd <jats:sup>2+</jats:sup> sensitivity and Cd <jats:sup>2+</jats:sup> accumulation in yeast. Furthermore, <jats:italic>AtNramp3</jats:italic> and <jats:italic>AtNramp4</jats:italic> complement an iron uptake mutant in yeast. This suggests possible roles in iron transport in plants and reveals heterogeneity in the functional properties of <jats:italic>Nramp</jats:italic> transporters. In <jats:italic>Arabidopsis</jats:italic> , <jats:italic>AtNramps</jats:italic> are expressed in both roots and aerial parts under metal replete conditions. Interestingly, <jats:italic>AtNramp3</jats:italic> and <jats:italic>AtNramp4</jats:italic> are induced by iron starvation. Disruption of the <jats:italic>AtNramp3</jats:italic> gene leads to slightly enhanced cadmium resistance of root growth. Furthermore, overexpression of <jats:italic>AtNramp3</jats:italic> results in cadmium hypersensitivity of <jats:italic>Arabidopsis</jats:italic> root growth and increased accumulation of Fe, on Cd <jats:sup>2+</jats:sup> treatment. Our results show that <jats:italic>Nramp</jats:italic> genes in plants encode metal transporters and that <jats:italic>AtNramps</jats:italic> transport both the metal nutrient Fe and the toxic metal cadmium. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 97 (9), 4991-4996, 2000-04-25
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1363107369615209216
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- NII論文ID
- 80011915891
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- ISSN
- 10916490
- 00278424
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- データソース種別
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