Formation of Well-Oriented Microtubules with Preferential Polarity in a Confined Space under a Temperature Gradient
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- Akira Kakugo
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Yoshiki Tamura
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Kazuhiro Shikinaka
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Momoko Yoshida
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Ryuzo Kawamura
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Hidemitsu Furukawa
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Yoshihito Osada
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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- Jian Ping Gong
- Department of Biological Science, Graduate School of Science, Hokkaido University, and PRESTO, Japan Science and Technology Agency, Sapporo 060-0810, Japan
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説明
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
収録刊行物
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- Journal of the American Chemical Society
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Journal of the American Chemical Society 131 (50), 18089-18095, 2009-11-23
American Chemical Society (ACS)