Structural Insights into Calicivirus Attachment and Uncoating

DOI Web Site 6 Citations
  • David Bhella
    Medical Research Council Virology Unit, Glasgow University, Church St., Glasgow G11 5JR, United Kingdom
  • Derek Gatherer
    Medical Research Council Virology Unit, Glasgow University, Church St., Glasgow G11 5JR, United Kingdom
  • Yasmin Chaudhry
    Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom
  • Rebecca Pink
    Medical Research Council Virology Unit, Glasgow University, Church St., Glasgow G11 5JR, United Kingdom
  • Ian G. Goodfellow
    Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom

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Description

<jats:title>ABSTRACT</jats:title> <jats:p> The <jats:italic>Caliciviridae</jats:italic> family comprises positive-sense RNA viruses of medical and veterinary significance. In humans, caliciviruses are a major cause of acute gastroenteritis, while in animals respiratory illness, conjunctivitis, stomatitis, and hemorrhagic disease are documented. Investigation of virus-host interactions is limited by a lack of culture systems for many viruses in this family. Feline calicivirus (FCV), a member of the <jats:italic>Vesivirus</jats:italic> genus, provides a tractable model, since it may be propagated in cell culture. Feline junctional adhesion molecule 1 (fJAM-1) was recently identified as a functional receptor for FCV. We have analyzed the structure of this virus-receptor complex by cryo-electron microscopy and three-dimensional image reconstruction, combined with fitting of homology modeled high-resolution coordinates. We show that domain 1 of fJAM-1 binds to the outer face of the P2 domain of the FCV capsid protein VP1, inducing conformational changes in the viral capsid. This study provides the first structural view of a native calicivirus-protein receptor complex and insights into the mechanisms of virus attachment and uncoating. </jats:p>

Journal

  • Journal of Virology

    Journal of Virology 82 (16), 8051-8058, 2008-08-15

    American Society for Microbiology

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