Attenuation in trabecular bone: A comparison between numerical simulation and experimental results in human femur

<jats:p>Numerical simulations (finite-difference time domain) are compared to experimental results of ultrasound wave propagation through human trabecular bones. Three-dimensional high-resolution microcomputed tomography reconstructions served as input geometry for the simulation. The numerical simulation took into account scattering, but not absorption. Simulated and experimental values of the attenuation coefficients (α, dB/cm) and the normalized broadband ultrasound attenuation (nBUA, dB/cm/MHz) were measured and compared on a set of 28 samples. While experimental and simulated nBUA values were highly correlated (R2=0.83), and showed a similar dependence with bone volume fraction, the simulation correctly predicted experimental nBUA values only for low bone volume fraction (BV/TV). Attenuation coefficients were underestimated by the simulation. The absolute difference between experimental and simulated α values increased with both BV/TV and frequency. As a function of frequency, the relative difference between experimental and simulated α values decreased from 60% around 400kHz to 30% around 1.2MHz. Under the assumption that the observed discrepancy expresses the effect of the absorption, our results suggests that nBUA and its dependence on BV/TV can be mostly explained by scattering, and that the relative contribution of scattering to α increases with frequency, becoming predominant (&gt;50 %) over absorption for frequencies above 600kHz.</jats:p>