STAYGREEN, STAY HEALTHY: a loss‐of‐susceptibility mutation in the <i>STAYGREEN</i> gene provides durable, broad‐spectrum disease resistances for over 50 years of US cucumber production
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- Yuhui Wang
- Horticulture Department University of Wisconsin Madison WI 53706 USA
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- Junyi Tan
- Horticulture Department University of Wisconsin Madison WI 53706 USA
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- Zhiming Wu
- Horticulture Department University of Wisconsin Madison WI 53706 USA
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- Kyle VandenLangenberg
- Horticultural Science Department North Carolina State University Raleigh NC 27695 USA
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- Todd C. Wehner
- Horticultural Science Department North Carolina State University Raleigh NC 27695 USA
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- Changlong Wen
- Beijing Vegetable Research Center Beijing Academy of Agricultural and Forestry Sciences Beijing 100097 China
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- Xiangyang Zheng
- Magnum Seeds Inc. Dixon CA 95620 USA
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- Ken Owens
- Magnum Seeds Inc. Dixon CA 95620 USA
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- Alyson Thornton
- HM Clause Seed Company Davis CA 95618 USA
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- Hailey H. Bang
- HM Clause Seed Company Davis CA 95618 USA
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- Eric Hoeft
- HM Clause Seed Company Davis CA 95618 USA
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- Peter A. G. Kraan
- Bayer Vegetable Seeds 6083 AB Nunhem the Netherlands
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- Jos Suelmann
- Bayer Vegetable Seeds 6083 AB Nunhem the Netherlands
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- Junsong Pan
- Horticulture Department University of Wisconsin Madison WI 53706 USA
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- Yiqun Weng
- Horticulture Department University of Wisconsin Madison WI 53706 USA
説明
<jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>The Gy14 cucumber (<jats:italic>Cucumis sativus</jats:italic>) is resistant to oomyceteous downy mildew (<jats:styled-content style="fixed-case">DM</jats:styled-content>), bacterial angular leaf spot (<jats:styled-content style="fixed-case">ALS</jats:styled-content>) and fungal anthracnose (<jats:styled-content style="fixed-case">AR</jats:styled-content>) pathogens, but the underlying molecular mechanisms are unknown.</jats:p></jats:list-item> <jats:list-item><jats:p>Quantitative trait locus (<jats:styled-content style="fixed-case">QTL</jats:styled-content>) mapping for the disease resistances in Gy14 and further map‐based cloning identified a candidate gene for the resistant loci, which was validated and functionally characterized by spatial‐temporal gene expression profiling, allelic diversity and phylogenetic analysis, as well as local association studies.</jats:p></jats:list-item> <jats:list-item><jats:p>We showed that the triple‐disease resistances in Gy14 were controlled by the cucumber <jats:italic><jats:styled-content style="fixed-case">STAYGREEN</jats:styled-content></jats:italic> (<jats:italic>Cs<jats:styled-content style="fixed-case">SGR</jats:styled-content></jats:italic>) gene. A single nucleotide polymorphism (<jats:styled-content style="fixed-case">SNP</jats:styled-content>) in the coding region resulted in a nonsynonymous amino acid substitution in the Cs<jats:styled-content style="fixed-case">SGR</jats:styled-content> protein, and thus disease resistance. Genes in the chlorophyll degradation pathway showed differential expression between resistant and susceptible lines in response to pathogen inoculation. The causal <jats:styled-content style="fixed-case">SNP</jats:styled-content> was significantly associated with disease resistances in natural and breeding populations. The resistance allele has undergone selection in cucumber breeding.</jats:p></jats:list-item> <jats:list-item><jats:p>The durable, broad‐spectrum disease resistance is caused by a loss‐of‐susceptibility mutation of <jats:italic>Cs<jats:styled-content style="fixed-case">SGR</jats:styled-content></jats:italic>. Probably, this is achieved through the inhibition of reactive oxygen species over‐accumulation and phytotoxic catabolite over‐buildup in the chlorophyll degradation pathway. The <jats:italic>Cs<jats:styled-content style="fixed-case">SGR</jats:styled-content></jats:italic>‐mediated host resistance represents a novel function of this highly conserved gene in plants.</jats:p></jats:list-item> </jats:list> </jats:p>
収録刊行物
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- New Phytologist
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New Phytologist 221 (1), 415-430, 2018-07-19
Wiley
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詳細情報 詳細情報について
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- CRID
- 1363107370265867392
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- ISSN
- 14698137
- 0028646X
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- Web Site
- https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.15353
- https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15353
- https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.15353
- https://nph.onlinelibrary.wiley.com/doi/am-pdf/10.1111/nph.15353
- https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15353
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