抄録
<jats:title>INTRODUCTION</jats:title><jats:p>The presence of polysaccharides in a DNA preparation can inhibit use of techniques such as polymerase chain reaction (PCR). Cetyltrimethyl ammonium bromide (CTAB) is a surfactant useful for isolation of DNA from tissues containing high amounts of polysaccharides. Under the high-salt conditions used in this protocol, the CTAB binds the polysaccharides, removing them from the solution. When used with <jats:italic>Arabidopsis</jats:italic>, this procedure yields quite pure DNA that is suitable for thermal asymmetric interlaced PCR (TAIL-PCR).</jats:p>
収録刊行物
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- Cold Spring Harbor Protocols
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Cold Spring Harbor Protocols 2009 (3), pdb.prot5177-, 2009-03
Cold Spring Harbor Laboratory
