In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs
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- Alexander N. Vaneev
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Petr V. Gorelkin
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Anastasiia S. Garanina
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Helena V. Lopatukhina
- Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia
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- Stepan S. Vodopyanov
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Anna V. Alova
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Oxana O. Ryabaya
- N. N. Blokhin National Medical Research Center of Oncology, 24 Kashirskoe shosse, Moscow 115478, Russia
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- Roman A. Akasov
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Yanjun Zhang
- Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China
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- Pavel Novak
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Sergey V. Salikhov
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Maxim A. Abakumov
- N. I. Pirogov Russian National Research Medical University, Ostrovityanova Street 1/7, Moscow 117997, Russia
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- Yasufumi Takahashi
- Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
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- Christopher R. W. Edwards
- Imperial College London, Department of Medicine, London W12 0NN, United Kingdom
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- Natalia L. Klyachko
- Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia
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- Alexander G. Majouga
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Yuri E. Korchev
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
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- Alexander S. Erofeev
- National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, Moscow 119049, Russia
説明
In vivo monitoring of reactive oxygen species (ROS) in tumors during treatment with anticancer therapy is important for understanding the mechanism of action and in the design of new anticancer drugs. In this work, a platinized nanoelectrode is placed into a single cell for detection of the ROS signal, and drug-induced ROS production is then recorded. The main advantages of this method are the short incubation time with the drug and its high sensitivity which allows the detection of low intracellular ROS concentrations. We have shown that our new method can measure the ROS response to chemotherapy in tumor-bearing mice in real-time. ROS levels were measured in vivo inside the tumor at different depths in response to doxorubicin. This work provides an effective new approach for the measurement of intracellular ROS by platinized nanoelectrodes.
収録刊行物
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- Analytical Chemistry
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Analytical Chemistry 92 (12), 8010-8014, 2020-05-22
American Chemical Society (ACS)
