Ethanol Vapor Is an Efficient Inducer of the <i>alc</i> Gene Expression System in Model and Crop Plant Species

DOI PDF 被引用文献2件
  • Justin P. Sweetman
    Crop Genetics Research, Syngenta, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, United Kingdom (J.P.S., A.J.G., I.J.); and
  • Chengcai Chu
    Institut fur Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, 06466 Gatersleben, Germany (C.C., N.Q., U.S.)
  • Nan Qu
    Institut fur Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, 06466 Gatersleben, Germany (C.C., N.Q., U.S.)
  • Andrew J. Greenland
    Crop Genetics Research, Syngenta, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, United Kingdom (J.P.S., A.J.G., I.J.); and
  • Uwe Sonnewald
    Institut fur Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, 06466 Gatersleben, Germany (C.C., N.Q., U.S.)
  • Ian Jepson
    Crop Genetics Research, Syngenta, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, United Kingdom (J.P.S., A.J.G., I.J.); and

抄録

<jats:title>Abstract</jats:title> <jats:p>We have demonstrated that low concentrations of ethanol vapor efficiently induce the alc gene expression system in tobacco (Nicotiana tabacum cv Samsun NN), potato (Solanum tuberosum cv Solara), and oilseed rape (Brassica napus cv Westar). For many situations, this may be the preferred method of induction because it avoids direct application of comparatively high concentrations of an ethanol solution. Although induction was seen with less than 0.4 μm ethanol vapor, maximal induction of the chloramphenicol acetyl transferase gene was achieved after 48 h in leaves of tobacco plants enclosed with 4.5 μm ethanol vapor. In the absence of ethanol, there is no detectable gene expression. Treatment of potato tubers with ethanol vapor results in uniform β-glucoronidase (GUS) expression. Vapor treatment of a single oilseed rape leaf resulted in induction of GUS in the treated leaf only and 14C-ethanol labeling in tobacco confirmed that the inducer was not translocated. In contrast, enclosure of the roots, aerial parts, or whole plant with ethanol vapor resulted in induction of GUS activity in leaves and roots. The data reported here broaden the utility of the alc system for research and crop biotechnology.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 129 (3), 943-948, 2002-07-01

    Oxford University Press (OUP)

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ