Identifying a large number of high-yield genes in rice by pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 gene knockout
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- Ju Huang
- State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, China;
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- Jing Li
- State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, China;
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- Jun Zhou
- Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138;
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- Long Wang
- State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, China;
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- Sihai Yang
- State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, China;
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- Laurence D. Hurst
- The Milner Centre for Evolution, University of Bath, BA2 7AY Bath, United Kingdom;
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- Wen-Hsiung Li
- Biodiversity Research Center, Academia Sinica, 115 Taipei, Taiwan;
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- Dacheng Tian
- State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, China;
抄録
<jats:title>Significance</jats:title> <jats:p>Finding the genes that control a complex trait is difficult because each gene may have only minor phenotypic effects. Quantitative trait loci mapping and genome-wide association study techniques have been developed for this purpose but are laborious and time-consuming. Here we developed a method combining pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 technology. By sequencing the parents and descendants of IR8, the Green Revolution “miracle rice,” we identified many genes that had been retained in the pedigree by selection for high yield. Knockout and knockdown studies showed that a large proportion of the identified genes are essential or have phenotypic effects related to production. Our approach provides a powerful means for identifying genes involved in a complex trait.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 115 (32), 2018-07-23
Proceedings of the National Academy of Sciences