Evaluation of the Most Probable Number Method for Enumerating Denitrifying Bacteria

DOI Web Site Web Site 被引用文献1件

書誌事項

公開日
1985-05
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.2136/sssaj1985.03615995004900030023x
公開者
Wiley

この論文をさがす

説明

<jats:title>Abstract</jats:title> <jats:p> Most probable number (MPN) enumerations of denitrifying bacteria were conducted on an agricultural soil (pH 6.6) and a forest soil (pH 5.6), in both neutral (pH 6.8) and acid (pH 5.6) media. The neutral medium yielded higher enumerations for both soils and, therefore, appears to be optimal, even for denitrifiers present in acid soils. Enzyme activity assays and MPN enumerations of seven soil samples were weakly correlated ( <jats:italic>r</jats:italic> = 0.71; <jats:italic>p</jats:italic> = 0.07). One soil exhibited a high MPN enumeration, but had no detectable denitrifying enzyme activity. While the enzyme assay indexes denitrification potential of soils under the environmental conditions at the time of sampling, the MPN enumeration indexes the denitrifying capability of the populations inhabiting soils. A population's denitrifying capability is related to its size, but other factors which might also influence MPN enumerations are discussed. Precise incubation time was critical in the MPN procedure. Delays of 2h affected the scoring results. Populations in some of the incubation tubes appeared to be in a dynamic growth phase at the end of the 14‐d incubation period. Inconsistencies in incubation duration could confound comparisons among soils. Despite inherent flaws and disadvantages, the MPN procedure may be appropriate for certain research objectives as an index of a population's denitrifying capacity. Recommendations and cautions are offered. </jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

問題の指摘

ページトップへ