Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease

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Description

<jats:title>Abstract</jats:title><jats:p>Human T cells coordinate adaptive immunity in diverse anatomic compartments through production of cytokines and effector molecules, but it is unclear how tissue site influences T cell persistence and function. Here, we use single cell RNA-sequencing (scRNA-seq) to define the heterogeneity of human T cells isolated from lungs, lymph nodes, bone marrow and blood, and their functional responses following stimulation. Through analysis of >50,000 resting and activated T cells, we reveal tissue T cell signatures in mucosal and lymphoid sites, and lineage-specific activation states across all sites including distinct effector states for CD8<jats:sup>+</jats:sup> T cells and an interferon-response state for CD4<jats:sup>+</jats:sup> T cells. Comparing scRNA-seq profiles of tumor-associated T cells to our dataset reveals predominant activated CD8<jats:sup>+</jats:sup> compared to CD4<jats:sup>+</jats:sup> T cell states within multiple tumor types. Our results therefore establish a high dimensional reference map of human T cell activation in health for analyzing T cells in disease.</jats:p>

Journal

  • Nature Communications

    Nature Communications 10 (1), 4706-, 2019-10-17

    Springer Science and Business Media LLC

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