Binding of Actinomycin Chromophore Analogues to DNA

DOI Web Site 被引用文献3件

書誌事項

公開日
1973-02-01
権利情報
  • http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining
DOI
  • 10.1139/o73-026
公開者
Canadian Science Publishing

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説明

<jats:p> Five chromophore analogues of actinomycin, viz. phenoxazone (1), aminophenoxazone (2), N-acetylaminophenoxazone (3), N-methylaminophenoxazone (4), and actinocin (5), were synthesized. Of these compounds, only 2 and 4 were found to interact with DNA. The binding constants for these compounds, obtained by spectrophotometric titration, were of the same order of magnitude as that obtained with actinomycin-DNA interaction. The major difference between the binding of actinomycin and of the analogues to DNA was the lability of the complex of the latter compounds in the presence of Mg<jats:sup>2+</jats:sup> ions. These observations have been interpreted to indicate intercalation of the chromophore analogues between successive base pairs of DNA. The spectra of actinocin were unaffected by the addition of DNA. The spectral changes indicative of an interaction between actinocin and DNA observed at low concentration of Mg<jats:sup>2+</jats:sup> ions were abolished at high concentrations of these ions. The implications of these observations to the mechanism of actinomycin binding to DNA are discussed. </jats:p>

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