Cell-free translation reconstituted with purified components
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説明
We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).
収録刊行物
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- Nature Biotechnology
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Nature Biotechnology 19 (8), 751-755, 2001-08
Springer Science and Business Media LLC
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詳細情報 詳細情報について
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- CRID
- 1363388843921984000
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- NII論文ID
- 80012411813
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- DOI
- 10.1038/90802
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- ISSN
- 15461696
- 10870156
- http://id.crossref.org/issn/10870156
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
- Integbio
