Extractive Solubilization, Structural Change, and Functional Conversion of Cytochrome <i>c</i> in Ionic Liquids via Crown Ether Complexation
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- Kojiro Shimojo
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
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- Noriho Kamiya
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
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- Fumito Tani
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
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- Hirochika Naganawa
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
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- Yoshinori Naruta
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
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- Masahiro Goto
- Division of Environment and Radiation Sciences, Nuclear Science and Engineering Directorate, Japan Atomic Energy Agency, Tokai-mura, Ibaraki, 319-1195, Japan, Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan, and Institute for Materials Chemistry and Engineering, Kyusyu University, Higashi-ku, Fukuoka 812-8581, Japan
書誌事項
- 公開日
- 2006-10-10
- DOI
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- 10.1021/ac0612877
- 公開者
- American Chemical Society (ACS)
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説明
This article reports on the extraction behavior of heme proteins from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the structure-function relationship of cytochrome c (Cyt-c) dissolved in ILs. We have found that DCH18C6 enables transfer of Lys-rich proteins into ILs via supramolecular complexation. The hydrophobicity and functional groups of ILs have a great influence on protein partitioning, and a hydroxyl group-containing IL with DCH18C6 is capable of the quantitative partitioning of Cyt-c. On the other hand, protein transfer using conventional organic solvents is negligibly small. UV-visible, CD, and resonance Raman spectroscopic characterizations indicate that the sixth ligand Met 80 in the heme group of the Cyt-c-DCH18C6 complex in IL is replaced by other amino acid residues of the peptide chain and that a non-natural, six-coordinate, low-spin ferric heme structure is induced in IL. Solubilization of Cyt-c in IL causes the environmental change of the heme vicinity of Cyt-c, which triggers the functional conversion of Cyt-c from an electron-transfer protein to peroxidase. The Cyt-c-DCH18C6 complex in IL provides remarkably high peroxidase activity compared with native Cyt-c, because of enhancement of the affinity for H2O2.
収録刊行物
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- Analytical Chemistry
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Analytical Chemistry 78 (22), 7735-7742, 2006-10-10
American Chemical Society (ACS)