Loss of Core Fucosylation of Low-Density Lipoprotein Receptor–Related Protein-1 Impairs Its Function, Leading to the Upregulation of Serum Levels of Insulin-Like Growth Factor–Binding Protein 3 in Fut8−/− Mice
Description
alpha1,6-Fucosyltransferase (Fut8) catalyzes the transfer of a fucose residue from GDP-fucose to the innermost N-acetylglucosamine residue of N-glycans. Here we report that the loss of core fucosylation impairs the function of low-density lipoprotein (LDL) receptor-related protein-1 (LRP-1), a multifunctional scavenger and signaling receptor, resulting in a reduction in the endocytosis of insulin like growth factor (IGF)-binding protein-3 (IGFBP-3) in the cells derived from Fut8-null (Fut8-/-) mice. The reduced endocytosis was restored by the re-introduction of Fut8. Serum levels of IGFBP-3 were markedly upregulated in Fut8-/- mice. These data clearly indicate that core fucosylation is crucial for the scavenging activity of LRP-1 in vivo.
Journal
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- The Journal of Biochemistry
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The Journal of Biochemistry 139 (3), 391-398, 2006-03-01
Oxford University Press (OUP)