<i>Bacillus subtilis</i> contains multiple Fur homologues: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors

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書誌事項

公開日
1998-07
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1046/j.1365-2958.1998.00921.x
公開者
Wiley

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説明

<jats:p>Fur (ferric uptake regulator) proteins control iron uptake in many Gram‐negative bacteria. Although Fur homologues have been identified in Gram‐positive bacteria, their roles in gene regulation are unknown. Genome sequencing has revealed three <jats:italic>fur</jats:italic> homologues in <jats:italic>Bacillus subtilis</jats:italic>: <jats:italic>yqkL</jats:italic>, <jats:italic>yqfV</jats:italic> and <jats:italic>ygaG</jats:italic>. We demonstrate that <jats:italic>yqkL</jats:italic> encodes an iron uptake repressor: both siderophore biosynthesis and transcription of ferri‐siderophore uptake genes is constitutive in the <jats:italic>yqkL</jats:italic> mutant. Thus, <jats:italic>yqkL</jats:italic> encodes a repressor that is functionally as well as structurally related to Fur. <jats:italic>B. subtilis</jats:italic> peroxide stress genes are induced by either H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> or by metal ion limitation. Previous genetic studies defined a regulatory locus, <jats:italic>perR</jats:italic>, postulated to encode the peroxide regulon repressor. We demonstrate that a <jats:italic>ygaG</jats:italic> mutant has the <jats:italic>perR</jats:italic> phenotype: it is highly resistant to peroxides and overexpresses catalase, alkyl hydroperoxide reductase and the DNA binding protein MrgA. Nine spontaneous <jats:italic>perR</jats:italic> mutations, isolated by virtue of their ability to derepress <jats:italic>mrgA</jats:italic> transcription in the presence of manganous ion, all contain sequence changes in the <jats:italic>ygaG</jats:italic> locus and can be complemented by the cloned <jats:italic>ygaG</jats:italic> gene. Thus, <jats:italic>ygaG</jats:italic> encodes the peroxide regulon repressor and is allelic with <jats:italic>perR</jats:italic>.</jats:p>

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