Upregulation of Inducible Nitric Oxide Synthase Contributes to Attenuated Cutaneous Vasodilation in Essential Hypertensive Humans

<jats:p> Essential hypertension is a proinflammatory, proconstrictor disease coinciding with endothelial dysfunction and inward vessel remodeling. Using the skin circulation, our aim was to determine whether inducible NO synthase (iNOS) upregulation attenuates NO-dependent cutaneous vasodilation in hypertensive humans. We hypothesized that, with hypertension, localized iNOS inhibition would restore vasodilation in response to NO-dependent stimuli, and iNOS expression would be increased and phosphorylated vasodilator-stimulated phosphoprotein would be decreased. For, in vivo protocols, 4 intradermal microdialysis fibers were placed in 9 hypertensive and 10 normotensive men and women (systolic blood pressure: 146±4 versus 113±2 mm Hg; <jats:italic>P</jats:italic> <0.001). Microdialysis fibers served as control, iNOS inhibited (1400 W), neuronal NO synthase inhibited ( <jats:italic> N <jats:sup>ω</jats:sup> - </jats:italic> propyl <jats:italic> - <jats:sc>l</jats:sc> </jats:italic> -arginine), and nonselective NOS inhibited ( <jats:italic>N</jats:italic> <jats:sup>G</jats:sup> -nitro- <jats:sc>l</jats:sc> -arginine methyl ester). Cutaneous vascular conductance was calculated (percentage of sodium nitroprusside) during standardized local heating (42°C) and acetylcholine dose-response protocols (0.01, 0.10, 1.00, 5.00, 10.00, 50.00, 100.00 mmol/L). The NO-dependent local heating response was attenuated at control (95±2% versus 76±2% cutaneous vascular conductance; <jats:italic>P</jats:italic> <0.05) and neuronal NO synthase–inhibited sites (94±4% versus 77±3% cutaneous vascular conductance; <jats:italic>P</jats:italic> <0.01) in hypertensives. iNOS inhibition augmented the NO-dependent local heating response (93±2% versus 89±10% cutaneous vascular conductance). Acetylcholine-induced vasodilation was attenuated in control sites at doses ≥0.1 mmol/L of acetylcholine in hypertensives and was restored with iNOS inhibition (0.1 mmol/L, <jats:italic>P</jats:italic> <0.05; 1, 5, and 10 mmol/L, <jats:italic>P</jats:italic> <0.001; 50 and 100 mmol/L, <jats:italic>P</jats:italic> <0.01). In vitro iNOS expression was increased ( <jats:italic>P</jats:italic> =0.006) and phosphorylated vasodilator-stimulated phosphoprotein was decreased in skin from hypertensive humans ( <jats:italic>P</jats:italic> =0.04). These data suggest that iNOS is upregulated in essential hypertensive humans and contributes to reduced NO-dependent cutaneous vasodilation. </jats:p>