Evaluation of an Ovine Testis Cell Line (OA3.Ts) for Propagation of Capripoxvirus Isolates and Development of an Immunostaining Technique for Viral Plaque Visualization

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  • Shawn Babiuk
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada
  • Geoff Parkyn
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada
  • John Copps
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada
  • June E. Larence
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada
  • Marta I. Sabara
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada
  • Timothy R. Bowden
    University of Manitoba, Department of Immunology, Basic Medical Sciences Building, 730 William Avenue, Winnipeg MB, R3E 0W3 Canada
  • David B. Boyle
    University of Manitoba, Department of Immunology, Basic Medical Sciences Building, 730 William Avenue, Winnipeg MB, R3E 0W3 Canada
  • R. Paul Kitching
    National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada

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<jats:p> An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (<33) and high (34−36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum. </jats:p>

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