3-Methylhistidine in actin and other muscle proteins

  • P. Johnson
    Department of Biochemistry, University of Birmingham
  • C I Harris
    Department of Biochemistry, University of Birmingham
  • S V Perry
    Department of Biochemistry, University of Birmingham

書誌事項

公開日
1967-10-01
DOI
  • 10.1042/bj1050361
公開者
Portland Press Ltd.

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説明

<jats:p>1. By the use of the extended elution system for basic amino acid analysis, 3-methylhistidine has been detected in hydrolysates of actin isolated from mammalian, fish and bird skeletal muscle. 2. Evidence is presented to indicate that 3-methylhistidine forms part of the primary structure and that in rabbit actin this residue is restricted to one peptide fraction obtained from the tryptic digest. 3. Rabbit skeletal-muscle actin has a 3-methylhistidine:histidine ratio 1:7·6, indicating a minimum molecular weight of 47600. 4. Adult rabbit myosin contains approximately 2 3-methylhistidine residues/mol. These residues are localized in the heavy meromyosin part of the molecule, and are restricted to the major component obtained after succinylation.</jats:p>

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