Evaluation of lophine derivatives as L‐012 (luminol analog)‐dependent chemiluminescence enhancers for measuring horseradish peroxidase and H<sub>2</sub>O<sub>2</sub>

DOI Web Site Web Site PubMed 被引用文献1件
  • T. Ichibangase
    Research Institute of Pharmaceutical Sciences Musashino University Japan
  • Y. Ohba
    Faculty of Pharmaceutical Sciences Nagasaki International University Japan
  • N. Kishikawa
    Graduate School of Biomedical Sciences Nagasaki University Japan
  • K. Nakashima
    Faculty of Pharmaceutical Sciences Nagasaki International University Japan
  • N. Kuroda
    Graduate School of Biomedical Sciences Nagasaki University Japan

書誌事項

公開日
2013-04-30
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/bio.2513
公開者
Wiley

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説明

<jats:title>ABSTRACT</jats:title><jats:p>8‐Amino‐5‐chloro‐7‐phenylpyrido[3,4‐d]pyridazine‐1,4(2H,3H)dione (L‐012) was recently synthesized as a new chemiluminescence (CL) probe; the light intensity and the sensitivity of L‐012 are higher than those of other CL probes such as luminol. Previously, our group developed four lophine‐based CL enhancers of the horseradish peroxidase (HRP)‐catalyzed CL oxidation of luminol, namely 2‐(4‐hydroxyphenyl)‐4,5‐diphenylimidazole (HDI), 2‐(4‐hydroxyphenyl)‐4,5‐di(2‐pyridyl)imidazole (HPI), 4‐(4,5‐diphenyl‐1H‐imidazol‐2‐yl)phenylboronic acid (DPA), and 4‐[4,5‐di(2‐pyridyl)‐1H‐imidazol‐2‐yl]phenylboronic acid (DPPA), and showed that DPPA was suitable for the photographic detection of HRP. In this study, we replaced luminol with L‐012 and evaluated these as L‐012‐dependent CL enhancers. In addition, to detect HRP and/or H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> with higher sensitivity, each detection condition for the L‐012–HRP–H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> enhanced CL was optimized. All the derivatives enhanced the L‐012‐dependent CL as well as luminol CL; HPI generated the highest enhanced luminescence. Under optimized conditions for HRP detection, the detection limit of HRP was 0.08 fmol. By contrast, the detection limit of HRP with the enhanced L‐012‐dependent CL using 4‐iodophenol, which is a common enhancer of luminol CL, was 1.1 fmol. With regard to H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> detection, the detection limits for enhanced CL with HPI and 4‐iodophenol were 0.29 and 1.5 pmol, respectively. Therefore, it is demonstrated that HPI is the most superior L‐012‐dependent CL enhancer. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.</jats:p>

収録刊行物

  • Luminescence

    Luminescence 29 (2), 118-121, 2013-04-30

    Wiley

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