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- Patrick Seitz
- Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Swiss Federal Institute of Technology Lausanne (École Polytechnique Fédérale de Lausanne), CH-1015 Lausanne, Switzerland
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- Melanie Blokesch
- Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Swiss Federal Institute of Technology Lausanne (École Polytechnique Fédérale de Lausanne), CH-1015 Lausanne, Switzerland
説明
<jats:title>Significance</jats:title> <jats:p> Transformation allows naturally competent bacteria to take up DNA from the environment and integrate the DNA into the chromosome by recombination. In Gram-negative bacteria, the DNA-uptake machinery shuttles the incoming DNA across the outer membrane, the periplasmic space, and the inner membrane. This study investigates the DNA-uptake complex of the human pathogen <jats:italic>Vibrio cholerae</jats:italic> , using a cellular biology-based approach. We visualized different components of this multicomponent complex, including a type IV pilus appendage, determined their (co)localization within the bacterial cell, and conducted an analysis of competence-gene mutants. We conclude that the uptake of DNA occurs via (at least) a two-step process. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 110 (44), 17987-17992, 2013-10-14
Proceedings of the National Academy of Sciences