Conserved Functions of Ikaros in Vertebrate Lymphocyte Development: Genetic Evidence for Distinct Larval and Adult Phases of T Cell Development and Two Lineages of B Cells in Zebrafish
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- Michael Schorpp
- Department of Developmental Immunology, Max-Planck Institute of Immunobiology , Freiburg ,
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- Mike Bialecki
- Department of Developmental Immunology, Max-Planck Institute of Immunobiology , Freiburg ,
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- Dagmar Diekhoff
- Department of Developmental Immunology, Max-Planck Institute of Immunobiology , Freiburg ,
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- Brigitte Walderich
- Exelixis Germany , Tübingen ,
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- Jörg Odenthal
- Exelixis Germany , Tübingen ,
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- Hans-Martin Maischein
- Department of Genetics, Max-Planck Institute of Developmental Biology , Tübingen ,
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- Agustin G Zapata
- Department of Cell Biology, Complutense University , Madrid ,
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- Thomas Boehm
- Department of Developmental Immunology, Max-Planck Institute of Immunobiology , Freiburg ,
書誌事項
- 公開日
- 2006-08
- 権利情報
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- https://academic.oup.com/pages/standard-publication-reuse-rights
- DOI
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- 10.4049/jimmunol.177.4.2463
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Zebrafish has been advocated as an alternative animal model to study lymphocyte development, although the similarities in the genetic requirements of lymphopoiesis between fish and mammals have not yet been investigated. In this study, we examine the role of the transcription factor Ikaros in zebrafish lymphopoiesis. In fish larvae homozygous for an ikaros allele predicted to lack the C-terminal zinc fingers, T lymphopoiesis is absent; the presence of VHDμJμ rearrangements in adolescent fish is delayed in mutants. In adolescent mutant fish, T cells expressing tcrb and tcrd and B cells expressing igm are formed with low efficiency and display an oligoclonal Ag receptor repertoire. By contrast, B cells expressing the igz isotype do not develop, providing genetic evidence for two separate B cell lineages in zebrafish. Thus, Ikaros appears to play similar roles in fish and mammalian lymphopoiesis.</jats:p>
収録刊行物
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- The Journal of Immunology
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The Journal of Immunology 177 (4), 2463-2476, 2006-08
Oxford University Press (OUP)