Transient overexpression of γ interferon promotes<i>Aspergillus</i>clearance in invasive pulmonary aspergillosis
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- C Shao
- Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, China
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- J Qu
- Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, China
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- L He
- Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, China
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- Y Zhang
- Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
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- J Wang
- Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
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- Y Wang
- Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
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- H Zhou
- Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, China
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- X Liu
- Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
説明
<jats:title>Summary</jats:title><jats:p>Cytokines are critical molecules necessary for normal lung pathogen host defences. Gamma interferon (IFN-γ) and T1-phenotype immune responses are important components of host defence against Aspergillus. Therefore, we hypothesized that transient overexpression of IFN-γ within the lung could augment host immunity against Aspergillus. Here it was showed that intranasal administration of 5 × 107 colony-forming units (CFU) of Aspergillus fumigatus (Af ) induced the expression of IFN-γ. Mice were intranasally (i.n) administrated with 5 × 108 PFU of a recombinant adenovirus vector containing the murine IFN-γ cDNA (AdmIFN-γ), and challenged 24 h later with Af. We observed that i.n. administration of AdmIFN-γ resulted in about a fourfold increase in levels of IFN-γ and IL-12 within the lung, about a 75% reduction in lung fungal contents at day 2 and a more than threefold higher survival rate in the AdmIFN-γ-treated group compared to the controls (P < 0·01). This protection effect was not found when AdmIFN-γ was i.p. administrated. Alveolar macrophages and lung leucocytes isolated from i.n. AdmIFN-γ-treated animals displayed enhanced killing of intracellular Aspergillus organisms ex vivo. These results demonstrate that transient overexpression of IFN-γ could augment host defence against Aspergillus.</jats:p>
収録刊行物
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- Clinical and Experimental Immunology
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Clinical and Experimental Immunology 142 (2), 233-241, 2005-09-22
Oxford University Press (OUP)