The Purification of Hepatitis‐Associated Antigen (HAA/SH/AU) from Human Serum and the Preparation of a Sheep Anti‐HAA Serum

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<jats:p><jats:bold>Abstract.</jats:bold> HAA was obtained from a pool of HAA‐containing sera. The purification consisted of the following steps:</jats:p><jats:p>Extraction with Freon, adsorption on and elution from Aerosil and gel filtration on a Sepharose 4B column.</jats:p><jats:p>After purification, the presence of the HAA was demonstrated in the macro‐Ouchterlony. The purity was tested by immunoelectrophoresis and double immunodiffusion.</jats:p><jats:p>Sheep were immunized with the purified HAA. In the macro‐Ouchterlony, the presence of anti‐HAA antibodies in the adsorbed sheep serum was demonstrated as a single precipitation line, identical with our human HAA anti‐HAA precipitation line.</jats:p><jats:p>The anti‐HAA sheep serum was tested with good results against the HAA panel of the National Institutes of Health, Bethesda, USA.</jats:p>

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