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- Jeffrey Combs
- *Department of Molecular Cell and Developmental Biology, University of Texas, 1 University Station, MC C0930, Austin, TX 78712;
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- Soo Jin Kim
- *Department of Molecular Cell and Developmental Biology, University of Texas, 1 University Station, MC C0930, Austin, TX 78712;
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- Sarah Tan
- *Department of Molecular Cell and Developmental Biology, University of Texas, 1 University Station, MC C0930, Austin, TX 78712;
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- Lee A. Ligon
- Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104; and
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- Erika L. F. Holzbaur
- Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104; and
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- Jeffrey Kuhn
- Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
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- Martin Poenie
- *Department of Molecular Cell and Developmental Biology, University of Texas, 1 University Station, MC C0930, Austin, TX 78712;
書誌事項
- 公開日
- 2006-10-03
- DOI
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- 10.1073/pnas.0600914103
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p>Binding of T cells to antigen-presenting cells leads to the formation of the immunological synapse, translocation of the microtubule-organizing center (MTOC) to the synapse, and focused secretion of effector molecules. Here, we show that upon activation of Jurkat cells microtubules project from the MTOC to a ring of the scaffolding protein ADAP, localized at the synapse. Loss of ADAP, but not lymphocyte function-associated antigen 1, leads to a severe defect in MTOC polarization at the immunological synapse. The microtubule motor protein cytoplasmic dynein clusters into a ring at the synapse, colocalizing with the ADAP ring. ADAP coprecipitates with dynein from activated Jurkat cells, and loss of ADAP prevents MTOC translocation and the specific recruitment of dynein to the synapse. These results suggest a mechanism that links signaling through the T cell receptor to translocation of the MTOC, in which the minus end-directed motor cytoplasmic dynein, localized at the synapse through an interaction with ADAP, reels in the MTOC, allowing for directed secretion along the polarized microtubule cytoskeleton.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 103 (40), 14883-14888, 2006-10-03
Proceedings of the National Academy of Sciences