The F-Box Protein ZEITLUPE Confers Dosage-Dependent Control on the Circadian Clock, Photomorphogenesis, and Flowering Time[W]

DOI PDF 被引用文献21件
  • David E. Somers
    Department of Plant Biology/Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210
  • Woe-Yeon Kim
    Department of Plant Biology/Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210
  • Ruishuang Geng
    Department of Plant Biology/Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210

抄録

<jats:title>Abstract</jats:title> <jats:p>As an F-box protein, ZEITLUPE (ZTL) is involved in targeting one or more substrates for ubiquitination and degradation via the proteasome. The initial characterization of ZTL suggested a function limited largely to the regulation of the circadian clock. Here, we show a considerably broader role for ZTL in the control of circadian period and photomorphogenesis. Using a ZTL-specific antibody, we quantitated and characterized a ZTL dosage series that ranges from a null mutation to a strong ZTL overexpressor. In the dark,ztl null mutations lengthen circadian period, and overexpression causes arrhythmicity, suggesting a more comprehensive role for this protein in the clock than previously suspected. In the light, circadian period becomes increasingly shorter at higher levels of ZTL, to the point of arrhythmicity. By contrast, hypocotyl length increases and flowering time is delayed in direct proportion to the level of ZTL. We propose a novel testable mechanism by which circadian period and amplitude may act together to gate phytochrome B–mediated suppression of hypocotyl. We also demonstrate that ZTL-dependent delay of flowering is mediated through decreases in CONSTANS and FLOWERING LOCUS T message levels, thus directly linking proteasome-dependent proteolysis to flowering.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 16 (3), 769-782, 2004-03-01

    Oxford University Press (OUP)

被引用文献 (21)*注記

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