The MinC component of the division site selection system in <i>Escherichia coli</i> interacts with FtsZ to prevent polymerization
-
- Zonglin Hu
- Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160
-
- Amit Mukherjee
- Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160
-
- Sebastien Pichoff
- Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160
-
- Joe Lutkenhaus
- Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160
書誌事項
- 公開日
- 1999-12-21
- DOI
-
- 10.1073/pnas.96.26.14819
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p> Positioning of the Z ring at the midcell site in <jats:italic>Escherichia coli</jats:italic> is assured by the <jats:italic>min</jats:italic> system, which masks polar sites through topological regulation of MinC, an inhibitor of division. To study how MinC inhibits division, we have generated a MalE-MinC fusion that retains full biological activity. We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity. MalE-MinC19 has reduced ability to inhibit division, reduced affinity for FtsZ, and reduced ability to inhibit FtsZ polymerization. These results, along with MinC localization, suggest that MinC rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 96 (26), 14819-14824, 1999-12-21
Proceedings of the National Academy of Sciences