The Structural Dynamics and Ligand Releasing Process after the Photodissociation of Sperm Whale Carboxymyoglobin

DOI Web Site 被引用文献9件
  • Masaki Sakakura
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 606-8501, Japan
  • Isao Morishima
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 606-8501, Japan
  • Masahide Terazima
    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 606-8501, Japan

書誌事項

公開日
2001-09-28
DOI
  • 10.1021/jp0116252
公開者
American Chemical Society (ACS)

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説明

The structural and energy dynamics and molecular diffusion after the photodissociation of sperm whale carboxymyoglobin were investigated by the transient grating (TG) method. The quantitative measurement of the TG signal provides the volume change and released heat within 10 ns (−5 cm3 mol-1 and 151 kJ mol-1, respectively) as well as with a lifetime of 700 ns at 20 °C (12 cm3 mol-1 and 8 kJ mol-1) without any assumption. From the signal intensity with a wide range of grating vector (105 to 8 × 106 m-1), the molecular origin of the 700-ns dynamics is clearly attributed to the ligand escaping process from the protein interior to the solvent. Hence, a four-state model is appropriate to describe the ligand dissociation reaction even at room temperature. Participation of the salt bridge between Arg45 and the heme 6-propionate side chain as well as the enthalpy change and the structural change of this photodissociation reaction is discussed.

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