Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer.
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- J C Huang
- Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.
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- D L Svoboda
- Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.
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- J T Reardon
- Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.
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- A Sancar
- Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.
書誌事項
- 公開日
- 1992-04-15
- DOI
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- 10.1073/pnas.89.8.3664
- 公開者
- Proceedings of the National Academy of Sciences
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説明
<jats:p>By using a human cell-free system capable of nucleotide excision repair, a synthetic substrate consisting of a plasmid containing four thymidine dimers at unique locations, and deoxyribonucleoside 5'-[alpha-thio]triphosphates for repair synthesis, we obtained DNA fragments containing repair patches with phosphorothioate linkages. Based on the resistance of these linkages to digestion by exonuclease III and their sensitivity to cleavage by I2, we were able to delineate the borders of the repair patch to single-nucleotide resolution and found an asymmetric patch with sharp boundaries. That the repair patch was produced by filling in a gap generated by an excision nuclease and not by nick-translation was confirmed by the finding that the thymidine dimer was released in a 27- to 29-nucleotide oligomer.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 89 (8), 3664-3668, 1992-04-15
Proceedings of the National Academy of Sciences
