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- P. Rajesh Kumar
- Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
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- Yao Yu
- Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
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- Rolf Sternglanz
- Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
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- Stephen Albert Johnston
- Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
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- Leemor Joshua-Tor
- Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
書誌事項
- 公開日
- 2008-02-22
- DOI
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- 10.1126/science.1151903
- 公開者
- American Association for the Advancement of Science (AAAS)
この論文をさがす
説明
<jats:p> Transcriptional regulation of the galactose-metabolizing genes in <jats:italic>Saccharomyces cerevisiae</jats:italic> depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS <jats:sub>GAL</jats:sub> ); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event. </jats:p>
収録刊行物
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- Science
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Science 319 (5866), 1090-1092, 2008-02-22
American Association for the Advancement of Science (AAAS)