An automated flinch detecting system for use in the formalin nociceptive bioassay
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- Tony L. Yaksh
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- George Ozaki
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- Damon McCumber
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- Michael Rathbun
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- Camilla Svensson
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- Shelle Malkmus
- Department of Anesthesiology, University of California, San Diego, La Jolla, California 92093; and
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- Michael C. Yaksh
- Yaksh Magnetic Solutions, Lilburn, Georgia 30047
抄録
<jats:p>The biphasic display of paw-flinch behavior in the rat after injection of formalin into the dorsum of the hind paw is used for the screening of anti-hyperalgesic agents. Described and characterized here is a less labor-intensive system for counting flinch activity by detecting movement of a small metal band placed on the formalin-injected paw. A signal is generated as the band breaks the electromagnetic field of a loop antenna located under the rat and processed through an algorithm that determines flinch activity using 1) amplitude, 2) zero-voltage crossing, and 3) signal duration. Flinches are summed and stored over a selected collection interval throughout the assay for later analysis. Studies have validated the measures with respect to 1) system stability over time; 2) system-to-“practiced observer” correlation on flinch detection, r<jats:sup>2</jats:sup>= 0.94; 3) system variables including time of day, sex, age, and body weight; and 4) 50% effective dose values similar to those previously reported for intrathecal morphine and the NMDA antagonist MK-801.</jats:p>
収録刊行物
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- Journal of Applied Physiology
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Journal of Applied Physiology 90 (6), 2386-2402, 2001-06-01
American Physiological Society