Single Nucleotide Recognition Using a Probes-On-Carrier DNA Chip
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- Saifullah
- Area of Bioscience & Biotechnology, School of Materials Science, Japan Advanced Institute of Science & Technology (JAIST), 1–1 Asahidai, Nomi, Ishikawa923–1292, Japan
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- Satoshi Fuke
- Area of Bioscience & Biotechnology, School of Materials Science, Japan Advanced Institute of Science & Technology (JAIST), 1–1 Asahidai, Nomi, Ishikawa923–1292, Japan
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- Hiroshi Nagasawa
- Kankyou Resilience, 79–7 Tokiwadai, Hodogaya, Yokohama, 240–0067, Japan
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- Toshifumi Tsukahara
- Area of Bioscience & Biotechnology, School of Materials Science, Japan Advanced Institute of Science & Technology (JAIST), 1–1 Asahidai, Nomi, Ishikawa923–1292, Japan
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説明
Following the sequencing of the human genome, SNP analysis of individual patients has become essential for achieving the best drug response and ensuring optimal care. In this study, we developed a cost-effective probes-on-carrier DNA chip for the detection of SNPs. Our chips harbored three different probes against the TP53 gene, and were capable of detecting wild-type TP53 and SNPs such as rs121912651 and rs11540652. Four cell lines were used to validate the specificity of probe hybridization. Strong fluorescence intensity was observed in hybridized spots based on hybridization for perfect base pairing between complementary strands, whereas significantly lower fluorescence (p 0.05) was observed in nonhybridized spots. These hybridization results indicated that the probes-on-carrier chip is suitable for SNP genotyping.
収録刊行物
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- BioTechniques
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BioTechniques 66 (2), 73-78, 2019-02
Informa UK Limited
