Microbore high-performance liquid chromatography and chemiluminescence detection of Dns-amino acids

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書誌事項

公開日
1984-01
権利情報
  • https://www.elsevier.com/tdm/userlicense/1.0/
DOI
  • 10.1016/s0021-9673(00)96178-8
公開者
Elsevier BV

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説明

Abstract Application of the chemiluminescence (CL) post-column reaction to microbore column (250 × 1 mm I.D., 10 μm) high-performance liquid chromatography is described for the sensitive detection of Dns-amino acids. Dns-alanine, -valine, -isoleucine and -phenylalanine, separated on a reversed-phase column, were detected with CL, generated by the reaction with bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide. The detection limits are of the order of 2 × 10 -16 mol (S/N  3) and the average deviation for the peak height of 10 fmol of each Dns-amino acid was 1.3% ( n  5).

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