Detection of a Low-Grade Enteroviral Infection in the Islets of Langerhans of Living Patients Newly Diagnosed With Type 1 Diabetes
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- Lars Krogvold
- Paediatric Department, Oslo University Hospital, Oslo, Norway
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- Bjørn Edwin
- Intervention Centre and Department of Surgery, Oslo University Hospital, Oslo, Norway
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- Trond Buanes
- Faculty of Medicine, University of Oslo, Oslo, Norway
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- Gun Frisk
- Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Oskar Skog
- Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Mahesh Anagandula
- Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Olle Korsgren
- Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Dag Undlien
- Faculty of Medicine, University of Oslo, Oslo, Norway
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- Morten C. Eike
- Department of Medical Genetics, Oslo University Hospital, Oslo, Norway
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- Sarah J. Richardson
- Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
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- Pia Leete
- Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
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- Noel G. Morgan
- Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
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- Sami Oikarinen
- Department of Virology, School of Medicine, University of Tampere, Tampere, Finland
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- Maarit Oikarinen
- Department of Virology, School of Medicine, University of Tampere, Tampere, Finland
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- Jutta E. Laiho
- Department of Virology, School of Medicine, University of Tampere, Tampere, Finland
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- Heikki Hyöty
- Department of Virology, School of Medicine, University of Tampere, Tampere, Finland
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- Johnny Ludvigsson
- Division of Paediatrics, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
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- Kristian F. Hanssen
- Faculty of Medicine, University of Oslo, Oslo, Norway
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- Knut Dahl-Jørgensen
- Paediatric Department, Oslo University Hospital, Oslo, Norway
抄録
<jats:p>The Diabetes Virus Detection study (DiViD) is the first to examine fresh pancreatic tissue at the diagnosis of type 1 diabetes for the presence of viruses. Minimal pancreatic tail resection was performed 3–9 weeks after onset of type 1 diabetes in six adult patients (age 24–35 years). The presence of enteroviral capsid protein 1 (VP1) and the expression of class I HLA were investigated by immunohistochemistry. Enterovirus RNA was analyzed from isolated pancreatic islets and from fresh-frozen whole pancreatic tissue using PCR and sequencing. Nondiabetic organ donors served as controls. VP1 was detected in the islets of all type 1 diabetic patients (two of nine controls). Hyperexpression of class I HLA molecules was found in the islets of all patients (one of nine controls). Enterovirus-specific RNA sequences were detected in four of six patients (zero of six controls). The results were confirmed in various laboratories. Only 1.7% of the islets contained VP1+ cells, and the amount of enterovirus RNA was low. The results provide evidence for the presence of enterovirus in pancreatic islets of type 1 diabetic patients, which is consistent with the possibility that a low-grade enteroviral infection in the pancreatic islets contributes to disease progression in humans.</jats:p>
収録刊行物
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- Diabetes
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Diabetes 64 (5), 1682-1687, 2014-11-24
American Diabetes Association