Zinc Salts Inactivate Clinical Isolates of Herpes Simplex Virus In Vitro

DOI Web Site 被引用文献1件
  • Max Arens
    <!--label omitted: 1-->The Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine,1 and
  • Sharon Travis
    <!--label omitted: 2-->St. Louis Children's Hospital,2 St. Louis, Missouri 63110

書誌事項

公開日
2000-05
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jcm.38.5.1758-1762.2000
公開者
American Society for Microbiology

この論文をさがす

説明

<jats:title>ABSTRACT</jats:title><jats:p>Using a standard plaque assay and clinical isolates of herpes simplex virus (HSV), we have tested the ability of zinc salts to inactivate HSV. Virus was treated by incubation at 37°C with zinc salts in morpholinepropanesulfonic acid-buffered culture medium and was then diluted and plated onto CV-1 cells for detection and quantitation of remaining infectious virus. Of 10 randomly chosen clinical isolates (five HSV type 1 [HSV-1] isolates and five HSV-2 isolates), seven were inactivated >98% by treatment in vitro with 50 mM zinc gluconate for 2 h and nine were inactivated >97% by treatment with zinc lactate. The effect was concentration dependent. With an HSV-1 isolate, 50 mM zinc gluconate or zinc lactate caused 100% inactivation, 15 mM caused 98 to 99% inactivation, and 5 mM caused 63 to 86% inactivation. With an HSV-2 isolate, 50 and 15 mM zinc gluconate caused 30% inactivation and 5 and 1 mM caused less than 9% inactivation, whereas 50 and 15 mM zinc lactate caused greater than 92% inactivation and 5 and 1 mM caused 37 and 26% inactivation, respectively. The ability of the zinc salts to inactivate HSV was not related to pH in the pH range of 6.1 to 7.6 since inactivation by zinc gluconate or zinc lactate in that pH range was 99.7 to 100% with a 2-h treatment with 50 mM zinc salt. Short (5-min) treatments of selected isolates with zinc gluconate, zinc lactate, zinc acetate, or zinc sulfate yielded inactivation rates of 0 to 55%.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ