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- Wilhelmine N. de Vries
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
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- Alexei V. Evsikov
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
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- Bryce E. Haac
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
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- Karen S. Fancher
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
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- Andrea E. Holbrook
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
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- Rolf Kemler
- Max-Planck Institute of Immunobiology, Stuebeweg 51, D-79108 Freiburg,Germany
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- Davor Solter
- Max-Planck Institute of Immunobiology, Stuebeweg 51, D-79108 Freiburg,Germany
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- Barbara B. Knowles
- The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
書誌事項
- 公開日
- 2004-09-15
- DOI
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- 10.1242/dev.01316
- 公開者
- The Company of Biologists
この論文をさがす
説明
<jats:p>The oocyte to embryo transition in metazoans depends on maternal proteins and transcripts to ensure the successful initiation of development, and the correct and timely activation of the embryonic genome. We conditionally eliminated the maternal gene encoding the cell adhesion molecule E-cadherin and partially eliminated the β-catenin gene from the mouse oocyte. Oocytes lacking E-cadherin, or expressing a truncated allele of β-catenin without the N-terminal part of the protein, give rise to embryos whose blastomeres do not adhere. Blastomere adhesion is restored after translation of protein from the wild-type paternal alleles: at the morula stage in embryos lacking maternal E-cadherin, and at the late four-cell stage in embryos expressing truncated β-catenin. This suggests that adhesion per se is not essential in the early cleavage stage embryos, that embryos develop normally if compaction does not occur until the morula stage, and that the zona pellucida suffices to maintain blastomere proximity. Although maternal E-cadherin is not essential for the completion of the oocyte-to-embryo transition, absence of wild-type β-catenin in oocytes does statistically compromise developmental success rates. This developmental deficit is alleviated by the simultaneous absence of maternal E-cadherin, suggesting that E-cadherin regulates nuclear β-catenin availability during embryonic genome activation.</jats:p>
収録刊行物
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- Development
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Development 131 (18), 4435-4445, 2004-09-15
The Company of Biologists