Promoter-creating mutations in <i>Pseudomonas putida</i> : A model system for the study of mutation in starving bacteria
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- Lagle Kasak
- Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, 23 Riia Street, EE2400 Tartu, Estonia
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- Rita Hõrak
- Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, 23 Riia Street, EE2400 Tartu, Estonia
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- Maia Kivisaar
- Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, 23 Riia Street, EE2400 Tartu, Estonia
Description
<jats:p> A novel experimental system to study mutation in starving bacteria was designed, relying on the activation of a promoterless phenol degradation operon of <jats:italic>Pseudomonas putida</jats:italic> . The Phe <jats:sup>+</jats:sup> (phenol-utilizing) mutants accumulated in the starving culture of <jats:italic>P. putida</jats:italic> in the presence of phenol but not in the absence of it. We ruled out the possibility that the absence of phenol eliminates Phe <jats:sup>+</jats:sup> mutants from the starving population. Sequence analysis of the Phe <jats:sup>+</jats:sup> mutants revealed that base substitutions, deletions, and insertion of Tn <jats:italic>4652</jats:italic> can result in creation of a sequence similar to the σ <jats:sup>70</jats:sup> -specific promoter consensus. One particular C → A transversion was predominant in the Phe <jats:sup>+</jats:sup> mutants that arose in the starving population under selection for phenol use. In contrast, various deletions were the most frequent Phe <jats:sup>+</jats:sup> mutants occurring in a culture growing without selection. The accumulation rate of the Phe <jats:sup>+</jats:sup> mutants on selective plates was found to be higher for bacteria plated from stationary-phase culture than that from exponentially growing cells. This suggests that some specific processes, occurring predominantly in stationary-phase cells, facilitate generation and/or fixation of such mutations. </jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 94 (7), 3134-3139, 1997-04
Proceedings of the National Academy of Sciences
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Details 詳細情報について
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- CRID
- 1363670318356660736
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- ISSN
- 10916490
- 00278424
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- Data Source
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- Crossref