Paired quantitative and qualitative assessment of the replication-competent HIV-1 reservoir and comparison with integrated proviral DNA
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- Julio C. C. Lorenzi
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Yehuda Z. Cohen
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Lillian B. Cohn
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Edward F. Kreider
- Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
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- John P. Barton
- Department of Chemical Engineering, Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA 02139;
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- Gerald H. Learn
- Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
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- Thiago Oliveira
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Christy L. Lavine
- Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215;
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- Joshua A. Horwitz
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Allison Settler
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Mila Jankovic
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Michael S. Seaman
- Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215;
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- Arup K. Chakraborty
- Department of Chemical Engineering, Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA 02139;
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- Beatrice H. Hahn
- Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
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- Marina Caskey
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
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- Michel C. Nussenzweig
- Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065;
説明
<jats:title>Significance</jats:title> <jats:p> A reservoir of latently infected cells poses the greatest challenge to HIV-1 eradication. Efforts to develop strategies to eliminate the reservoir have been hampered, in part, by the lack of a precise understanding of the cellular and molecular nature of this reservoir. We describe a new method to analyze the replication-competent latent reservoir quantitatively and qualitatively. We find that over 50% of the replication-competent viruses in the reservoir form part of groups with identical <jats:italic>env</jats:italic> sequences. However, a negative correlation exists between integrated proviral clones and replication-competent viruses, such that the larger the proviral clone, the lower is its probability of representing a replication-competent virus. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 113 (49), E7908-, 2016-11-21
Proceedings of the National Academy of Sciences