Structure of the human gastric bacterial community in relation to <i>Helicobacter pylori</i> status
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- Ana Maldonado-Contreras
- Department of Biology, University of Puerto Rico , San Juan, PR , USA
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- Kate C Goldfarb
- Ecology Department, Earth Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA , USA
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- Filipa Godoy-Vitorino
- Department of Biology, University of Puerto Rico , San Juan, PR , USA
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- Ulas Karaoz
- Ecology Department, Earth Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA , USA
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- Mónica Contreras
- Venezuelan Institute for Scientific Research (IVIC), Altos del Pipe , Miranda , Venezuela
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- Martin J Blaser
- Departments of Medicine and Microbiology, New York University Langone Medical Center , New York, NY , USA
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- Eoin L Brodie
- Ecology Department, Earth Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA , USA
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- Maria G Dominguez-Bello
- Department of Biology, University of Puerto Rico , San Juan, PR , USA
書誌事項
- 公開日
- 2010-10-07
- 権利情報
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- https://academic.oup.com/pages/standard-publication-reuse-rights
- DOI
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- 10.1038/ismej.2010.149
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>The human stomach is naturally colonized by Helicobacter pylori, which, when present, dominates the gastric bacterial community. In this study, we aimed to characterize the structure of the bacterial community in the stomach of patients of differing H. pylori status. We used a high-density 16S rRNA gene microarray (PhyloChip, Affymetrix, Inc.) to hybridize 16S rRNA gene amplicons from gastric biopsy DNA of 10 rural Amerindian patients from Amazonas, Venezuela, and of two immigrants to the United States (from South Asia and Africa, respectively). H. pylori status was determined by PCR amplification of H. pylori glmM from gastric biopsy samples. Of the 12 patients, 8 (6 of the 10 Amerindians and the 2 non-Amerindians) were H. pylori glmM positive. Regardless of H. pylori status, the PhyloChip detected Helicobacteriaceae DNA in all patients, although with lower relative abundance in patients who were glmM negative. The G2-chip taxonomy analysis of PhyloChip data indicated the presence of 44 bacterial phyla (of which 16 are unclassified by the Taxonomic Outline of the Bacteria and Archaea taxonomy) in a highly uneven community dominated by only four phyla: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Positive H. pylori status was associated with increased relative abundance of non-Helicobacter bacteria from the Proteobacteria, Spirochetes and Acidobacteria, and with decreased abundance of Actinobacteria, Bacteroidetes and Firmicutes. The PhyloChip detected richness of low abundance phyla, and showed marked differences in the structure of the gastric bacterial community according to H. pylori status.</jats:p>
収録刊行物
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- The ISME Journal
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The ISME Journal 5 (4), 574-579, 2010-10-07
Oxford University Press (OUP)