Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a

DOI Web Site 被引用文献19件
  • Bernard H. Ramsahoye
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom
  • Detlev Biniszkiewicz
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom
  • Frank Lyko
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom
  • Victoria Clark
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom
  • Adrian P. Bird
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom
  • Rudolf Jaenisch
    Department of Hematology, Western General Hospital, EH4 2XU Edinburgh, United Kingdom; Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142; and University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, Kings Buildings, Mayfield Road, EH9 3JR Edinburgh, United Kingdom

書誌事項

公開日
2000-05-09
DOI
  • 10.1073/pnas.97.10.5237
公開者
National Academy of Sciences

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<jats:p> Current evidence indicates that methylation of cytosine in mammalian DNA is restricted to both strands of the symmetrical sequence CpG, although there have been sporadic reports that sequences other than CpG may also be methylated. We have used a dual-labeling nearest neighbor technique and bisulphite genomic sequencing methods to investigate the nearest neighbors of 5-methylcytosine residues in mammalian DNA. We find that embryonic stem cells, but not somatic tissues, have significant cytosine-5 methylation at CpA and, to a lesser extent, at CpT. As the expression of the <jats:italic>de novo</jats:italic> methyltransferase Dnmt3a correlates well with the presence of non-CpG methylation, we asked whether Dnmt3a might be responsible for this modification. Analysis of genomic methylation in transgenic <jats:italic>Drosophila</jats:italic> expressing Dnmt3a reveals that Dnmt3a is predominantly a CpG methylase but also is able to induce methylation at CpA and at CpT. </jats:p>

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